| NUKLEONIKA 2008, 53(4):145-149
DNA DAMAGE IN SUBPOPULATIONS OF HUMAN LYMPHOCYTES IRRADIATED WITH DOSES IN THE RANGE OF 0-1 Gy OF X-RADIATIONMaria Wojewódzka1, Eugeniusz K. Machaj2,3, Aneta Goździk2, Teresa Iwaneńko1, Tomasz Ołdak4, Marcin Kruszewski1, Zygmunt Pojda 2,3 1 Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland 2 Department of Experimental Haematology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, 5 W. K. Roentgena Str., 02-781 Warsaw, Poland 3 Department of Radiobiology, Military Institute of Hygiene and Epidemiology, 128 Szaserów Str., 00-909 Warsaw, Poland 4 Polish Stem Cell Bank, 2/41 Grzybowska Str., 00-131 Warsaw, Poland We compared three methods usually applied in biological dosimetry for estimation of radiation-induced DNA damage in human T and B lymphocytes: alkaline comet assay, micronucleus (MN) test and formation of histone gamma-H2AX foci. Human peripheral blood lymphocytes were fractionated using T cells and B cells isolation kits. Cells were irradiated with doses in the range of 0–1 Gy of X-rays. Induction of DNA damage was assessed by the standard alkaline comet assay, MN test and histone gamma-H2AX foci immunofluorescence assay. Notwithstanding different end-points measured by the applied methods, all tests revealed a similar induction of DNA damage in B lymphocytes as compared with T lymphocytes. The results indicated that all three tests detect DNA damage with similar sensitivity, the lowest dose being approximately 0.3 Gy. The difference between irradiated and control cells was expressed as the ratio of the value obtained for irradiated cells (1 Gy) to that for control cells. The highest ratio was obtained for formation of gamma-H2AX foci and was 6.2 for T and 13.8 for B lymphocytes, whereas those for comet assay and micronucleus test were 3.5; 3.6 and 5.6; 4.8, respectively. Close X |