| NUKLEONIKA 2008, 53(4):161-165
GENOTOXIC EFFECTS OF 60Co GAMMA RAYS ON CHINESE
HAMSTER OVARY (CHO) CELLS
Tiberius Dicu1, Ioana Brie2, Piroska Virag2, Eva Fischer2, Maria Perde2, Vasile Foriº2, Valentin Cernea2,
Constantin Cosma1
1 Faculty of Environmental Science, “Babes-Bolyai” University,
4 ªtefan cel Mare, Cluj-Napoca, 400192, Romania
2 Cancer Institute “I. Chiricuþã”, 34/36 Republicii Str., Cluj-Napoca, 400015, Romania
The aim of the present study was to evaluate the cellular radiosensitivity and radiation-induced DNA damage and repair in Chinese hamster ovary (CHO) cells. Cell survival after irradiation was assessed using the clonogenic assay. The initial, radio-induced and residual DNA damage in Chinese hamster ovary (CHO) cells exposed to 60Co gamma rays were determined using the alkaline comet assay. A linear-quadratic (LQ) survival curve was observed in CHO line. Data obtained by comet assay demonstrated a linear dose-response correlation in the range of tested doses
(0.3–4 Gy). The process of DNA repair was modeled by exponential equation. In addition, we found a good correlation (R2 = 0.995) between clonogenic cell survival and radio-induced DNA damage.
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