| NUKLEONIKA 2009, 54(3):175-179
PREPARATION, QUALITY CONTROL AND BIODISTRIBUTION STUDIES
OF [61Cu]-OXINATE FOR PET TUMOR IMAGING
Amir R. Jalilian1, Samaneh Zolghadri2, Reza Faghihi2, Hassan Yousefnia1,
Javad Garousi1, Kamaledin Shafaii1, Fatemeh Bolourinovin1
1 Agricultural, Medical and Industrial Research School (AMIRS-NSTRI),
Karaj, Iran, P. O. Box 31485-498
2 Department of Nuclear Engineering, School of Engineering, Shiraz University,
Shiraz, Iran, P. O. Box 71345-3490
Targeting apoptosis is an interesting issue in molecular imaging and various modalities have been presented. However, recent experiences in nuclear pharmacy demonstrated the application of small tracer molecules is more desired. This work was conducted for production of a radiolabeled copper complex, i.e. Cu-oxinate as a potential PET tracer for apoptosis imaging in oncology. Cu-61 was prepared by natural zinc target irradiation with 22 MeV protons (150 ľA) via the natZn(p, xn)61Cu nuclear reaction with a yield of 3.33 mCi/ľAh. In order to obtain the best labeling method, optimization reactions were performed for pH, temperature and concentration followed by solid phase extraction. Biodistribution of the tracer was studied in wild-type and fibrosarcoma bearing mice. Under the optimized conditions, radio-thin-layer chromatography (RTLC) and HPLC showed radiochemical purities of 99.99% and 97% respectively (with a minimum specific activity of 16 Ci/mM). Biodistribution of the tracer in fibrosarcoma bearing mice demonstrated a significant tumor uptake after 3 h. Tumor:blood and tumor:muscle ratios were 2.0 and 6.0 after 3 h, respectively.
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