NUKLEONIKA 2009, 54(4):279-284

 


PREPARATION AND BIODISTRIBUTION OF 99mTc-IgG-HYNIC
IN NORMAL RATS



Saeed Rajabifar1, Mehdi Akhlaghi1, Amir R. Jalilian1, Fateme Bolourinovin1,
Behbood Maashkar2, Mahbobe Talebimehrdar3, Mahdieh Ghafouri4

1 Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS),
Moazen Blvd., Rajaeeshahr, P. O. Box 31485-498, Karaj, Iran

2 Engineering College, Research and Science Unit, Azad University, Tehran, Iran
3 Payam Noor University, Karaj, Iran
4 Arak University, Arak, Iran


Human gamma globulin can be labeled by a direct or indirect method of radiotracer incorporated in a protein molecule. In this indirect method hydrazinonicotinic acid (HYNIC) is used which saves the structure and biological activity of the protein. Our goal was the efficient labeling of the human gamma globulin and evaluation of its biodistribution in different organs which can be used on experimentally induced infection causing inflammation. Immune globulin is mixed with s-hynic and IgG-hynic is developed using sidle A-lyzer and stored at 20C which can be used at least for six months and then Sn-tricine kit is prepared which is used for 99mTc labeling. Efficiency of 99mTc-IgG-hynic labeling at pH 6.4 was very much dependent on ligand (hynic) and coligand (tricine) presence in the reaction mixture. Radiochemical purity was more than 90% in the kits prepared. Serum stability study showed no decomposition of 99mTc from the complex. The biodistribution studies showed the highest percentage ID/organ in the blood, liver and kidney, respectively. A human gamma globulin was successfully labeled through hynic to 99mTc by an indirect method with high radiochemical purity.


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