NUKLEONIKA 2011, 56(1):29-33

 


TREATMENT WITH SILVER NANOPARTICLES DELAYS REPAIR OF X-RAY INDUCED DNA DAMAGE IN HepG2 CELLS



Maria Wojewódzka1, Anna Lankoff1,2, Maria Dusińska3, Gunnar Brunborg4,
Jolanta Czerwińska5, Teresa Iwaneńko1, Tomasz Stępkowski1, Irena Szumiel1,
Marcin Kruszewski1,6

1 Center of Radiobiology and Biological Dosimetry,
Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland
2 Jan Kochanowski University, 5 Żeromskiego Str., 25-369 Kielce, Poland
3 Norwegian Institute for Air Research, 18 Instituttveien Str., 2027 Kjeller, Norway
4 Norwegian Institute of Public Health, 8 Lovisenbergg Str., 0456 Oslo, Norway
5 Warsaw University, 1 Miecznikowa Str., 02-096 Warsaw, Poland
6 Institute of Agricultural Medicine, 2 Jaczewskiego Str., 20-090 Lublin, Poland

Nanoparticles (NPs) defined as particles having at least one dimension below 100 nm have been applied in the last decade in industry and medicine. Recently, there is an increased concern about the biohazard aspect of the presence of NP in consumer goods and in the environment. Silver NP (Ag NP) cause oxidative stress in mammalian cells in result of generation of reactive oxygen species (ROS). This results in genotoxicity and mutagenicity, disturbed mitochondrial respiration, slowed proliferation and cell death. Using the alkaline comet assay, we examined the effect of combined treatment with Ag NP 20 nm or 200 nm and X-rays (2 Gy) in HepG2 cells. In addition, combined treatment with X-rays and titanium dioxide NP (TiO2 NP) 21 nm was also studied. No effect of NP pre-treatment on X-ray induced initial deoxyribonucleic acid (DNA) damage levels was observed for all three NP. In contrast, Ag NP treatment preceding exposure to X-rays caused a marked decrease in the rate of single strand break rejoining. The effect was particularly strong for Ag NP 20 nm. TiO2 NP pre-treatment had no effect on DNA repair.

Close X